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The DPBS with WBCs was collected into a 50 mL centrifuge tube

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Research indicates that puerarin enhances antioxidant defense by regulating ferritin, activates the Nrf2 signaling pathway through upregulation of GPX4 and SLC7A11, and effectively alleviates iron overload and lipid peroxidation in heart failure models, thereby protecting cardiomyocytes from damage [228]