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Immunoblot assays Cell lysates from HCC-1806, PC3 WT and PC3 GGT1 + cells were obtained by placing PBS-washed cell plates on ice and scraping with RIPA buffer (Thermo Scientific, 89900) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280)

L-carnitine also improves muscle recovery, reduces the accumulation of lactic acid, and increases the lactate threshold

A skin-restoring growth factor that supports skin repair GHK-Cu or Copper Tripeptide-1, is a naturally occurring growth factor made of three amino acids bonded to a copper ion

The tissues were then processed using standard paraffin embedding techniques, involving dehydration with increasing ethanol concentrations, clearing with xylene, and embedding in paraffin wax at 60C