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therefore it is unclear why TMAO was found increased in E3 mice both peripherally and in the brain compared to E4 mice

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In the zona glomerulosa of the adrenal cortex, activation of AT 1 R triggers the release of mineralocorticoids [149], which elevate blood pressure primarily via stimulation of sodium reabsorption and expansion of plasma volume, and secondarily via non-genomic mineralocorticoid receptor (MR)-mediated actions [155]