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This conformational change was observed in comparison of crystal structures of partially reduced SmTGR (PDB 2X8C) and the SmTGRNADP(H) complex (PDB 2X99) and results in a stacked arrangement for the FAD, NADPH and Y296 phenol (Figure 2) (Figure 7C it was determined that the first species to be captured with SVD deconvolution of the SmTGR Y296A variant reductive half-reaction was that of the SmTGRNADPH complex (inset)

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