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Although this tool was successfully used for the analysis of secondary structure in globular proteins, it was pointed out that extrapolating empirical Raman parameters from globular proteins to natively unfolded proteins will presumably lead to significant errors, as some of the features that necessarily typify globular proteins are not present in natively unfolded proteins (Maiti et al., 2004)

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Finally, the current evidence does not support an important role for increased permeability in bile acid diarrhea