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The MTHFD1L encoded enzyme is a mono-functional enzyme with 10-formyl-THF synthetase activity and the MTHFD2L encoded enzyme is a bi-functional enzyme with 5,10-methylene-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities

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This indicates that the Grx activity, presumably catalyzed by the N-terminal Grx domain of the enzyme [11], could be dissociated from the other activities of the enzyme, which should be catalyzed by the C-terminal GCUG motif

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