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Among the more than 20 identified aflatoxins, AFB 1 is the most toxic and has been classified as Group I carcinogen by the International Agency for Research on Cancer (IARC), due to its hepatoxicity, nephrotoxicity, immunotoxicity, teratogenicity, and mutagenicity [3,4,5,6]
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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight
It's a synthetic peptide consisting of 15 amino acids, derived from a larger protective protein naturally found in human gastric juice
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