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second, as a substrate for the production of hydrogen sulphide (H 2 S), which feeds the mitochondrial electron transfer chain and mediates per-sulphidation of ATPase and glycolytic enzymes, thereby stimulating cellular bioenergetics

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ALT, AST, ALP, in LO2 cell were monitored using assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)