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Among these methods, measuring the light intensity and color variations provided straightforward mechanisms for accurately detecting and assaying analytes in LOCs 11 The light/color-based signal readout techniques (optical methods) can be implemented into LOCs using different components, including light sources (e.g., laser rays, visible, infrared, or ultraviolet rays at a wide range of wavelengths) 12 , detectors (e.g., light intensity sensors, red/green/blue (RGB) sensors, spectrophotometers, etc .) 13 , and mirrors in both on-chip and off-chip configurations 14

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