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Lukacs RU, Goldstein AS, Devon A Lawson DA, Cheng D & Witte ON

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Firstly, roGFP-iL was expressed as described above for 16 h at 20 C, cells were harvested, washed in HEPES buffer (40 mM, pH 7.4) and adjusted to an OD 600 of 1.0 in HEPES buffer