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The nine tissue systems now documented in the preclinical literature were not predicted by computational models or receptor-binding assays at the outset they were identified sequentially as researchers in different laboratories independently tested the compound against different experimental injury models and consistently found meaningful effects

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This broader tissue distribution is particularly relevant for researchers designing cell-type-specific binding or functional assays, as the off-target receptor panel for retatrutide is wider than for either semaglutide or tirzepatide