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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

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To optimize absorption and minimize local irritation, it is recommended to rotate injection sites, such as the upper arms, thighs, and areas around the navel

Zinc is essential for immune function, wound healing, and taste perception, the last of which is already affected by the altered appetite signals from GLP-1 therapy