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The cells were blocked with 5% goat serum, 0.02% Triton X-100 in PBS for 30 min and then incubated for 12 h in phospho-Met (Tyr1234/1235) (D26) XP rabbit mAb diluted 1:1,000 in 1% goat serum in PBS at 4 C

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Limit the impact of systemic insults including hyperkalemia and hypermagnesia