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TR fluorescence was serially read with a Flexstation 3 (Molecular Devices), with 340 nm excitation, 520 nm (cut-off 495 nm) and 620 nm (cut-off 570 nm) emission, 400 s delay and 1500 s integration time

Furthermore, it highlights the role of NAD + in mitochondrial homeostasis in aging and disease conditions, the latest results of NAD + -boosting therapeutics in clinical trials, and areas of further translational development

Further doseresponse and steady-state administration studies will be required to optimize the dosing schedule for future trials to evaluate therapeutic efficacy