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Astrocytic activation is a hallmark of brain aging, resulting in astrogliosis and the release of pro-inflammatory mediators, including interleukin-6 (IL-6) and interleukin-1 (IL-1) (Knox et al., 2022)

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Site rotation best practices: Alternate between left and right thighs with each injection Move the injection site within the thigh area systematically (e.g., clockwise pattern) Avoid injecting into areas with scars, moles, or lesions Do not inject into areas with visible lipohypertrophy or lipoatrophy Regularly inspect injection sites for changes in skin texture or appearance The angle of injection is equally important for proper technique