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Moreover, estrogen has positive effects on the viability of skin fibroblasts, and by inducing the expression of transforming growth factor-beta (TGF) and tissue inhibitor of matrix metalloproteinases (TIMPs), and inhibiting matrix metalloproteinases (MMPs), E2 stimulates the production and maintenance of extracellular matrix components, such as collagen type I and III and fibrillin (Lephart and Naftolin, 2021)