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The formation of the covalent vinyl thioether bond resulted in stabilization of the otherwise transient covalent acyl-enzyme intermediate formed during catalysis of proteases/isopeptidases, enabling us to obtain crystals for SnCE1-SUMO1-PA (Fig

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Due to these risks, SbV is not recommended for HIV-VL co-infected patients as they experience higher toxicity and reduced efficacy (Haldar et al., 2011)