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It carries the strongest evidence base because most published protocols describe this exact schedule

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Hair and nail germ formation and elongation assay For the formation and elongation assay of hair and nail germs, primary skin dermal fibroblasts, skin epidermal keratinocytes, hair follicle dermal papilla cells, and nail fibroblasts (onychofibroblasts) were obtained from patients under ethical considerations

Some users prefer 1 mL for a more concentrated solution or 2.5 mL for finer dose granularity the trade-off is unit-count precision vs