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Polyclonal glial fibrillary acidic protein (GFAP) was purchased from Dako (Carpinteria, CA)

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Histotripsy is a non-invasive, non-thermal, mechanical process that uses a focused beam of sonic energy to destroy cancerous liver tumors and is currently in a non-randomized, prospective clinical trial to evaluate the efficacy and safety of the device for the treatment of primary or metastatic tumors located in the liver

doi: 10.3389/fped.2025.1648282 Received 17 June 2025 Accepted 22 October 2025 Published 18 November 2025 Volume 13 - 2025 Edited by Ronan Lordan, University of Pennsylvania, United States Reviewed by Alex Staffler, Central Teaching Hospital of Bolzano/Bozen, Italy Anne Chiaramello, George Washington University, United States Updates Copyright 2025 Tan, Tewani, Anand, Kong, Rajadurai and Chandran